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DNA molecular engineered diagnosis and treatment drugs are a kind of drugs with diagnostic and therapeutic functions by using the programmable, self-assembling, recognizable and catalytic characteristics of DNA molecules. DNA molecular engineering for the diagnosis and treatment of drugs can be accurately designed and synthesized to form DNA nanostructures with specific structures and functions, such as DNA tetrahedra, DNA origami, DNA tiles, etc. These DNA nanostructures can be functionalized with other molecules or nanoparticles to achieve targeted identification and regulation of cells, genes, drugs, etc., thus showing broad application prospects in biosensing, drug delivery, bioimaging, gene therapy and other fields.